This may give a tentative decision in designing a method for initial or examination experiments, which can be further modified or updated to produce a method which fits the separation system for better benefits concerning reproducibility, quantification, and many others. Solubility profile
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The mixture moves throughout the column at different velocities and interacts Together with the sorbent, also called the stationary phase. The velocity of each part from the combination depends upon 1) its chemical mother nature, 2) the nature of your column and 3) the composition of the mobile stag